Sheila A. Lukehart
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Sheila A. Lukehart
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The Lukehart laboratory studies the pathogenesis of syphilis and the immune response to Treponema pallidum in humans and in animal models. Our current major interest is the 12-membered tpr gene family of T. pallidum, which is hypothesized to encode surface-exposed antigens that are major targets of the protective immune response, may be involved in immune evasion, and are promising vaccine candidates. We have demonstrated that one member of the Tpr family, TprK, undergoes antigenic variation; studies related to the immunological relevance and molecular mechanism of this variation are ongoing. New studies are focused on TprC and TprD, which are also surface exposed, and which differ in sequence among T. pallidum strains and subspecies. The laboratory is also working to identify surface molecules that are targets of opsonization and to define the kinetics of and requirements for bactericidal activity by macrophages. Many of the projects described above involve collaborations with Drs. Arturo Centurion and Lorenzo Giacani.
Additionally, our laboratory is involved in studies of clinical aspects of syphilis. With Dr. Christina Marra (Neurology), the laboratory is exploring the molecular basis for neuroinvasion, the immunologic response to T. pallidum within the CNS, and the efficacy of recommended therapy for CNS syphilis in immunocompetent and HIV-infected patients. Other ongoing studies involve the investigation of emerging macrolide resistance in T. pallidum, application of a molecular typing method for T. pallidum to epidemiological studies of syphilis, and studies of yaws in Papua New Guinea.
Recent Publications from PubMed
- Molini B, Fernandez MC, Godornes C, Vorobieva A, Lukehart SA, Giacani LFrontiers in immunology. 2022 ; 13 : 862491
- Lieberman NAP, Lin MJ, Xie H, Shrestha L, Nguyen T, Huang ML, Haynes AM, Romeis E, Wang QQ, Zhang RL, Kou CX, Ciccarese G, Dal Conte I, Cusini M, Drago F, Nakayama SI, Lee K, Ohnishi M, Konda KA, Vargas SK, Eguiluz M, Caceres CF, Klausner JD, Mitjà O, Rompalo A, Mulcahy F, Hook EW, Lukehart SA, Casto AM, Roychoudhury P, DiMaio F, Giacani L, Greninger ALPLoS neglected tropical diseases. 2021 12; 15 12: e0010063
- Beale MA, Marks M, Cole MJ, Lee MK, Pitt R, Ruis C, Balla E, Crucitti T, Ewens M, Fernández-Naval C, Grankvist A, Guiver M, Kenyon CR, Khairullin R, Kularatne R, Arando M, Molini BJ, Obukhov A, Page EE, Petrovay F, Rietmeijer C, Rowley D, Shokoples S, Smit E, Sweeney EL, Taiaroa G, Vera JH, Wennerås C, Whiley DM, Williamson DA, Hughes G, Naidu P, Unemo M, Krajden M, Lukehart SA, Morshed MG, Fifer H, Thomson NRNature microbiology. 2021 12; 6 12: 1549-1560
- Lin MJ, Haynes AM, Addetia A, Lieberman NAP, Phung Q, Xie H, Nguyen TV, Molini BJ, Lukehart SA, Giacani L, Greninger ALPLoS neglected tropical diseases. 2021 09; 15 9: e0009753
- Marra CM, Maxwell CL, Sahi SK, Tantalo LC, Dunaway SB, Lukehart SAClinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2022 03; 74 4: e1-e5