Samuel I. Miller
Dr. Miller holds a B.A. from Johns Hopkins University and a M.D. from Baylor College of Medicine. Dr. Miller is a former faculty member of the Harvard Medical School. In addition to his Microbiology Department appointment, he is a Professor in the Department of Medicine, Division of Allergy and Infectious Diseases, and in the Department of Genome Sciences. He is an Adjunct Professor of Immunology. He is a past Director of a Research Center of Excellence in Biodefense and Emerging Infectious Diseases for the region and Director of the Cystic Fibrosis Research and Development Program at the University of Washington.
The lab is organized into research groups focusing on the study of:
1) Salmonellae translocated effectors (which are delivered across the phagosome membrane and function to modify host processes by binding host GTPases, and acting as glycerol-cholesterol acyltransferases and ubiquitin ligases.
2) Bioinformatic analysis, DNA sequencing and phenotypic comparison of Shigella and Salmonella outbreak strains from diverse geographic locations including Central America and Africa and specifically the emergence of non-typhoidal Salmonella strains in Africa.
3) The environmental remodeling of the gram-negative bacterial envelope that occurs when bacteria infect host tissues and specifically the regulated alteration of glycerolphospholipids in the bacterial outer membrane by using a combination of bacterial genetics and mass spectrometry analysis of lipids.
4) Using NMR and crystallography to define structural mechanisms by which PhoQ responds to antimicrobial peptides and low pH and specifically testing the hypothesis that signal transduction involves the loss of structure on one side of the membrane with specific gain of structure on the other side of the membrane.
5) Identification of human genome polymorphisms that alter susceptibility to bacterial infection and diversity of human immune responses by using the human cellular genome wide association study (GWAS) platform using bacteria and the HapMap cell collection of normal human diversity.
6) How the bacterial second messenger di-c-GMP controls cellular processes and use of a FRET based biosensor for c-di-GMP to understand bacterial cellular function and diversity, and development of compounds that alter human interferon signaling by competing with mammalian molecules that bind cyclic nucleotides.
7) Human studies that involve analysis of the microbiota. The diseases analyzed using a metagenomic approach and DNA sequencing include inflammatory bowl disease and cystic fibrosis, as well as stool transplantation.
Recent Publications from PubMed
- Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity.Kolodziejek AM, Altura MA, Fan J, Petersen EM, Cook M, Brzovic PS, Miller SICell reports. 2019 May; 27 7: 2147-2156.e5
- Cyclic-di-GMP regulation promotes survival of a slow-replicating subpopulation of intracellular Typhimurium.Petersen E, Mills E, Miller SIProceedings of the National Academy of Sciences of the United States of America. 2019 03; 116 13: 6335-6340
- Human and Extracellular DNA Depletion for Metagenomic Analysis of Complex Clinical Infection Samples Yields Optimized Viable Microbiome Profiles.Nelson MT, Pope CE, Marsh RL, Wolter DJ, Weiss EJ, Hager KR, Vo AT, Brittnacher MJ, Radey MC, Hayden HS, Eng A, Miller SI, Borenstein E, Hoffman LRCell reports. 2019 Feb; 26 8: 2227-2240.e5
- Kamischke C, Fan J, Bergeron J, Kulasekara HD, Dalebroux ZD, Burrell A, Kollman JM, Miller SIeLife. 2019 Jan; 8 :
- Chaudhary A, Kamischke C, Leite M, Altura MA, Kinman L, Kulasekara H, Blanc MP, Wang G, Terhorst C, Miller SIScience signaling. 2018 Nov; 11 558: