David N. Fredricks
1. The Human Microbiome: What factors determine the constituents of the human genital tract, and how do microbial communities on mucosal surfaces impact health? We use molecular and cultivation approaches to study the ecology of human microbial communities with a focus on the common but poorly understood condition bacterial vaginosis (BV). BV is the most common cause of vaginal discharge and is associated with numerous adverse health outcomes such as HIV infection and preterm birth. Why do some women develop BV, and why are relapse rates high after antibiotic therapy? Our projects span the scale from studies of individual bacterial species interacting with cultured vaginal epithelial cells in the lab, to population studies of women and their partners exploring shifts in microbial communities in response to host physiology or behaviors.
2. Pathogen Discovery: Many microbes have not been successfully cultivated in the laboratory, and therefore molecular cultivation-independent methods are needed to detect them. We develop and deploy nucleic acid sequence-based methods for discovering novel pathogens linked to human disease. We have discovered several novel bacterial species linked with the condition bacterial vaginosis using this molecular approach. We are searching for novel pathogens in several idiopathic human diseases.
3. Molecular Diagnostics: We seek to improve the detection of known human pathogens by developing sensitive and specific diagnostic tests. These assays rely on the detection of microbial nucleic acid sequences in human tissues or body fluids. Our main focus is the use of PCR for the diagnosis of fungal infections in cancer patients, but we also develop assays for detecting other pathogen groups such as bacteria and viruses.
Recent Publications from PubMed
- Antibiotic Exposure Prior to Respiratory Viral Infection is Associated with Progression to Lower Respiratory Tract Disease in Allogeneic Hematopoietic Cell Transplant Recipients.Ogimi C, Krantz EM, Golob JL, Waghmare A, Liu C, Leisenring WM, Woodard CR, Marquis S, Kuypers JM, Jerome KR, Pergam SA, Fredricks DN, Sorror ML, Englund JA, Boeckh MBiology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation. 2018 May; :
- Parallel detection of lactobacillus and bacterial vaginosis-associated bacterial DNA in the chorioamnion and vagina of pregnant women at term.Lannon SMR, Adams Waldorf KM, Fiedler T, Kapur RP, Agnew K, Rajagopal L, Gravett MG, Fredricks DNThe journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians. 2018 Mar; : 1-9
- Evaluation of the association between the concentrations of key vaginal bacteria and the increased risk of HIV acquisition in African women from five cohorts: a nested case-control study.McClelland RS, Lingappa JR, Srinivasan S, Kinuthia J, John-Stewart GC, Jaoko W, Richardson BA, Yuhas K, Fiedler TL, Mandaliya KN, Munch MM, Mugo NR, Cohen CR, Baeten JM, Celum C, Overbaugh J, Fredricks DNThe Lancet. Infectious diseases. 2018 May; 18 5: 554-564
- Seasonal clustering of sinopulmonary mucormycosis in patients with hematologic malignancies at a large comprehensive cancer center.Sivagnanam S, Sengupta DJ, Hoogestraat D, Jain R, Stednick Z, Fredricks DN, Hendrie P, Whimbey E, Podczervinski ST, Krantz EM, Duchin JS, Pergam SAAntimicrobial resistance and infection control. 2017 ; 6 : 123
- Associations between improvement in genitourinary symptoms of menopause and changes in the vaginal ecosystem.Mitchell CM, Srinivasan S, Plantinga A, Wu MC, Reed SD, Guthrie KA, LaCroix AZ, Fiedler T, Munch M, Liu C, Hoffman NG, Blair IA, Newton K, Freeman EW, Joffe H, Cohen L, Fredricks DNMenopause (New York, N.Y.). 2018 May; 25 5: 500-507