Impact of common human diversity in cellular pathways of importance to immunity and autoimmunity. Using cellular GWAS approaches, we are studying the variability in autophagy and inflammasome pathways that influence human response to infectious agents; drugs and vaccines and susceptibility to autoimmune diseases. Our recent work on human diversity in CD244 and its effects on cellular autophagy reflects this effort.
Host-Pathogen Interactions. We have previously developed cell-based assays to study protein-protein interactions in the host, using split-Gaussia luciferase and split-GFP approaches. We thus analyzed the interactions of Brucella melitensis TIR-like protein (TcpB) with host adaptor proteins, and found that TcpB interacts with the death domain (DD) of MyD88. Salmonella translocates effector proteins into the host cell, thereby modulating host function. We are studying interactions and host responses mediated by specific Salmonella typhimurium type III effector proteins.
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